Huang Group: Publications Latest

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  1. The receptor repertoire and functional profile of follicular T cells in HIV-infected lymph nodes,” Ben S. Wendel, Daniel Del Alcazar, Chenfeng He, Perla M. Del Río-Estrada, Benjamas Aiamkitsumrit, Yuria Ablanedo-Terrazas, Stefany M. Hernandez, Ke-Yue Ma, Michael Betts, Laura Pulido, Jun Huang, Phyllis A. Gimotty, Gustavo Reyes-Terán, Ning Jiang, Laura F. Su. Science Immunology, 2018

    Overview

    Follicular helper CD4+ T cells (TFH) play an integral role in promoting B cell differentiation and affinity maturation. While TFH cell frequencies are increased in lymph nodes (LN) from individuals infected with HIV, humoral immunity remains impaired during chronic HIV infection.  Whether HIV inhibits TFH responses in LNs remains unclear.  Advances in this area have been limited by the difficulty of accessing human lymphoid tissues.  Here, we combined high-dimensional mass cytometry with TCR repertoire sequencing to interrogate the composition of TFH cells in primary human LNs.  We found evidence for intact antigen-driven clonal expansion of TFH cells and selective utilization of specific CDR3 motifs during chronic HIV infection, but the resulting TFH cells acquired an activation-related TFH cell signature characterized by IL-21 dominance.  These IL-21+ TFH cells contained an oligoclonal HIV-reactive population, preferentially accumulated in patients with severe HIV infection, and associated with aberrant B cell distribution in the same LN.  These data indicate that TFH cells remain capable of responding to HIV antigens during chronic HIV infection but become functionally skewed and oligoclonally restricted under persistent antigen stimulation. 

  2. “T cell co-stimulatory receptor CD28 is a primary target for PD-1–mediated inhibition,” Hui E, Cheung J, Zhu J, Su X, Taylor MJ, Wallweber HA, Sasmal DK, Huang J, Kim JM, Mellman I, Vale RD. Science, 2017

  3. “Detection, phenotyping and quantification of antigen-specific T cells using a peptide-MHC dodecamer,” J. Huang, X. Zeng, N. Sigal, P. J. Lund, L.F. Su, H. Huang, Chien, S. and M. M. Davis. PNAS, 2016

  4. “A single peptide-major histocompatibility complex ligand triggers digital cytokine secretion in CD4+ T cells,” J. Huang, M. Brameshuber, X. Zeng, J. Xie, Q. Li, Y. Chien, S. Valitutti, and M.M. Davis. Immunity, 39, 836-857, 2013

  5. “γδ T cells recognize common B cell antigens to initiate IL-17 response,” X. Zeng, Y. Wei, J. Huang, E.W. Newell, B.A. Kidd, M.S. Kuhns, C.T. Weaver, M.M. Davis, and Y. Chien. Immunity, 37, 524-534, 2012

  6. “Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be 'preferentially' transported to the cSMAC,” J. Xie, J.B. Huppa, E.W. Newell, J. Huang, P.J. Ebert, Q.J. Li, and M.M. Davis. Nature Immunology, 13, 674-680, 2012

  7. “Two-stage cooperative T cell receptor-peptide major histocompatibility complex-CD8 trimolecular interactions amplify antigen discrimination,” N. Jiang*, J. Huang*, L.J. Edwards, B. Liu, Y. Zhang, C.D. Beal, B.D. Evavold, and C. Zhu. Immunity, 34, 13-23, 2011

  8. “The kinetics of two-dimensional TCR and pMHC interactions determine T-cell responsiveness.,” J. Huang, V.I. Zarnitsyna, B. Liu, L.J. Edwards, N. Jiang, B.D Evavold, and C. Zhu. Nature, 464, 932-936, 2010

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